|
KMID : 0379119830110010054
|
|
Korean Journal of Mycology
1983 Volume.11 No. 1 p.54 ~ p.55
|
|
|
Ergoline Alkaloid Production by Protoplast Fusion of Ergot Fungi
|
|
|
|
|
Abstract
|
|
|
Strain improvement of industrially important fungi such as the ergot fungus which have no sexual reproduction in cultures is possible through mutation and selection as well as somatic recombination via the parasexual cycle. Apparently the limiting factor in the parasexul cycle is the formation of a heterokaryon through hyphal anastomosis. However, this can be manipulated by forming heterokaryons through protoplast fusion. Lytic enzymes from the snail, Helik pomatia were used for the formation of protoplasts of strains of the fungus carrying auxotrophic deficiencies to serve as markers for the isolation of fusion hybrids.
Several attempts to improve the alkaloid production of the ergot fungus. strain SD 58 based on mutation and selection techniques were made. N-methyl-N´-nitro-N-nitrosoguanidine for mutation and a color reaction with Salkowski´s reagent for selection were used. A two-fold increase of alkaloid production and improved vegetative development of the strains were obtained from already high producing strains. A 60 percent increase of alkaloid production was also obtained from some of the auxotrophic revertant strains.
In the case of protoplast fusion an intraspecific fusion of strain SD 58 resulted in a more than four-fold increase of alkaloid in one hybrid. Hybrids had multinucleated cells which were not the normal state for strain SD 58. Sclerotialike cell structures which were found only in high producing hybrids and/or the heterokaryotic state might he responsible for the high alkaloid productivity. Some hybrids lacked the alkaloid induction effect by tryptophan which is pronounced regulatory mechanism well documented ire strain SD 58.
Hybrids of an ergotamine producing strain of Claviceps purpurea and strain SD 58, showed an ability to produce both clavine and peptide alkaloids but in less amount compared with the parent strains. Alkaloid induction by tryptophan in the hybrids showed a slight effect indicating that the regulatory mechanisms of alkaloid production in the hybrids were influenced by both parents, since tryptophan inhibits alkaloid production in the ergotamine parent strain.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
 
|